Dual role of the σ factor in primer RNA synthesis by bacterial RNA polymerase
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چکیده
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Primer-dependent synthesis by poliovirus RNA-dependent RNA polymerase (3Dpol)
Properties of poliovirus RNA-dependent RNA polymerase (3D(pol)) including optimal conditions for primer extension, processivity and the rate of dissociation from primer-template (k(off)) were examined in the presence and absence of viral protein 3AB. Primer-dependent polymerization was examined on templates of 407 or 1499 nt primed such that fully extended products would be 296 or 1388 nt, resp...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 2018
ISSN: 0014-5793,1873-3468
DOI: 10.1002/1873-3468.13312